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STEMCELL Technologies Inc bit 9500 serum substitute
Bit 9500 Serum Substitute, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum+substitute+bit+9500/bit+9500/bio_rxiv__2025__07__15__664865-166-25-33
Average 90 stars, based on 1 article reviews
bit 9500 serum substitute - by Bioz Stars, 2026-10
90/100 stars

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Cell Culture:

Article Title: Multi-objective optimization reveals time- and dose-dependent inflammatory cytokine-mediated regulation of human stem cell derived T-cell development
Article Snippet: .. Unless otherwise described, cells were cultured in Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 μg/ml low-density lipoprotein (Stemcell Technologies), 60 μM ascorbic acid (Sigma), 24 μM 2-mercaptoethanol (Sigma), and 1% penicillin-streptomycin (Invitrogen). ..

Article Title: LncRNA IRAIN overcomes imatinib resistance in chronic myeloid leukemia via NF-κB/CD44 pathway inhibition
Article Snippet: The CD34 + cells were immunomagnetically enriched according to the instructions of the EasySep CD34 Positive Selection Kit (STEMCELL Technologies), and their purity was detected using a FITC-labeled anti-CD34 antibody by flow cytometry. .. As described elsewhere, the enriched CD34 + and mononuclear cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with Serum Substitute BIT 9500 (STEMCELL Technologies), 10 -4 M 2-mercaptoethanol, 20 ng/mL IL-3, 20 ng/mL IL-6, 100 ng/mL Flt3-ligand, and 20 ng/mL granulocyte colony-stimulating factor (STEMCELL Technologies), in a humidified atmosphere at 37°C, with 5% CO 2 . .. The human cell lines K562, MEG-01, U937, MV4-11, and HEK293T were purchased from the American Type Culture Collection.

Article Title: Avermectins Inhibit Replication of Parvovirus B19 by Disrupting the Interaction Between Importin α and Non-Structural Protein 1.
Article Snippet: PBMCs were isolated using centrifugation in Ficoll-Paque Plus (GE Healthcare Bio-Sciences AB, Milan, Italy) and cultured in a medium containing erythropoietic growth and differentiation factors, following previously established protocols with minor modifications [11]. .. Isolated PBMCs were cultured in IMDM supplemented with 20% serum substitute BIT 9500 (StemCell Technologies, Vancouver, BC, Canada) and enriched with 900 ng/mL ferrous sulfate, 90 ng/mL ferric nitrate, 1 μM hydrocortisone (#H0888, Merck Millipore, Milan, Italy), 3 U/mL Epoetin beta (NeoRecormon, Roche, Monza, Italy), 5 ng/mL IL-3 and 20 ng/mL stem cell factor (Thermo Fisher Scientific, Monza, Italy). ..

Article Title: Coordinated β-globin expression and α2-globin reduction in a multiplex lentiviral gene therapy vector for β-thalassemia.
Article Snippet: .. Human CD34+ transduction and culture Human CD34+ cells were cultured in StemPro34 SFM (serum free medium, Thermo Fisher Scientific) supplemented with the human cytokines fms-related tyrosine kinase 3 ligand (hFlt3L), Stem Cell Factor (hSCF), and interleukin-3 (hIL-3) (at 50, 50, and 10 ng/mL, respectively; all from Miltenyi Biotech) in the presence of 20% serum substitute (BIT 9500, STEMCELL Technologies). ..

Article Title: Antiviral activity of brincidofovir on parvovirus B19.
Article Snippet: .. PBMC, isolated by centrifugation in Ficoll-Paque Plus (GE Healthcare Bio-Sciences AB), were cultured in IMDM (Gibco) supplemented with 20% serum substitute BIT 9500 (StemCell Technologies), and enriched with erythropoietic growth factors as described (Bua et al., 2016; Filippone et al., 2010). ..

Article Title: Sex biased human thymic architecture guides T cell development through spatially defined niches
Article Snippet: .. Cells were cultured in Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 μg/ml low density lipoprotein (Stemcell Technologies), 60 μM ascorbic acid (Sigma), 24 μM 2-mercaptoethanol (Sigma), and 1% penicillin-streptomycin (Invitrogen). ..

Article Title: CD33-directed immunotherapy with third-generation chimeric antigen receptor T cells and gemtuzumab ozogamicin in intact and CD33-edited acute myeloid leukemia and hematopoietic stem and progenitor cells.
Article Snippet: The 293T cells were cultured in Iscove's Modified Dulbecco's Medium (IMDM; Thermo Fisher Scientific) containing 10% FBS. .. CD34-positive HSPCs were cultured in IMDM containing 20% serum substitute BIT 9500 (Stem Cell Technologies, Vancouver, Canada), 50 ng/mL recombinant human thrombopoietin (TPO; Miltenyi Biotec), 100 ng/ mL stem cell factor (SCF; Miltenyi Biotec), 100 ng/mL—FLT3-ligand (FLT3-L, Miltenyi Biotec), 100 μM beta-mercaptoethanol (Thermo Fisher Scientific), 50 μg/mL gentamicin (Carl Roth, Karlsruhe, Germany), 1 penicillin/streptomycin (pen/strep; Biochrom) and 35 nM UM171 (Miltenyi Biotec). .. AML blasts were cultured in IMDM (Thermo Fisher Scientific) containing 15% BIT 9500, 20 ng/mL interleukin (IL)-3 (Miltenyi Biotec), 20 ng/mL granulocyte colonystimulating factor (G-CSF) (Miltenyi Biotec), 50 ng/mL FLT3-L, 100 ng/mL SCF, 50 μg/mL gentamicin, 10 μg/mL ciprofloxacin (Carl Roth), 100 μM beta-Mercaptoethanol, 500 nM stemregenin 1 (SR1, Stem Cell Technologies) and 500 nM UM729 (Miltenyi Biotec).

Modification:

Article Title: Multi-objective optimization reveals time- and dose-dependent inflammatory cytokine-mediated regulation of human stem cell derived T-cell development
Article Snippet: .. Unless otherwise described, cells were cultured in Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 μg/ml low-density lipoprotein (Stemcell Technologies), 60 μM ascorbic acid (Sigma), 24 μM 2-mercaptoethanol (Sigma), and 1% penicillin-streptomycin (Invitrogen). ..

Article Title: LncRNA IRAIN overcomes imatinib resistance in chronic myeloid leukemia via NF-κB/CD44 pathway inhibition
Article Snippet: The CD34 + cells were immunomagnetically enriched according to the instructions of the EasySep CD34 Positive Selection Kit (STEMCELL Technologies), and their purity was detected using a FITC-labeled anti-CD34 antibody by flow cytometry. .. As described elsewhere, the enriched CD34 + and mononuclear cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with Serum Substitute BIT 9500 (STEMCELL Technologies), 10 -4 M 2-mercaptoethanol, 20 ng/mL IL-3, 20 ng/mL IL-6, 100 ng/mL Flt3-ligand, and 20 ng/mL granulocyte colony-stimulating factor (STEMCELL Technologies), in a humidified atmosphere at 37°C, with 5% CO 2 . .. The human cell lines K562, MEG-01, U937, MV4-11, and HEK293T were purchased from the American Type Culture Collection.

Article Title: Sex biased human thymic architecture guides T cell development through spatially defined niches
Article Snippet: .. Cells were cultured in Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 μg/ml low density lipoprotein (Stemcell Technologies), 60 μM ascorbic acid (Sigma), 24 μM 2-mercaptoethanol (Sigma), and 1% penicillin-streptomycin (Invitrogen). ..

Isolation:

Article Title: Avermectins Inhibit Replication of Parvovirus B19 by Disrupting the Interaction Between Importin α and Non-Structural Protein 1.
Article Snippet: PBMCs were isolated using centrifugation in Ficoll-Paque Plus (GE Healthcare Bio-Sciences AB, Milan, Italy) and cultured in a medium containing erythropoietic growth and differentiation factors, following previously established protocols with minor modifications [11]. .. Isolated PBMCs were cultured in IMDM supplemented with 20% serum substitute BIT 9500 (StemCell Technologies, Vancouver, BC, Canada) and enriched with 900 ng/mL ferrous sulfate, 90 ng/mL ferric nitrate, 1 μM hydrocortisone (#H0888, Merck Millipore, Milan, Italy), 3 U/mL Epoetin beta (NeoRecormon, Roche, Monza, Italy), 5 ng/mL IL-3 and 20 ng/mL stem cell factor (Thermo Fisher Scientific, Monza, Italy). ..

Article Title: Antiviral activity of brincidofovir on parvovirus B19.
Article Snippet: .. PBMC, isolated by centrifugation in Ficoll-Paque Plus (GE Healthcare Bio-Sciences AB), were cultured in IMDM (Gibco) supplemented with 20% serum substitute BIT 9500 (StemCell Technologies), and enriched with erythropoietic growth factors as described (Bua et al., 2016; Filippone et al., 2010). ..

Transduction:

Article Title: Coordinated β-globin expression and α2-globin reduction in a multiplex lentiviral gene therapy vector for β-thalassemia.
Article Snippet: .. Human CD34+ transduction and culture Human CD34+ cells were cultured in StemPro34 SFM (serum free medium, Thermo Fisher Scientific) supplemented with the human cytokines fms-related tyrosine kinase 3 ligand (hFlt3L), Stem Cell Factor (hSCF), and interleukin-3 (hIL-3) (at 50, 50, and 10 ng/mL, respectively; all from Miltenyi Biotech) in the presence of 20% serum substitute (BIT 9500, STEMCELL Technologies). ..

Purification:

Article Title: Ascorbate Plus Buformin in AML: A Metabolic Targeted Treatment.
Article Snippet: .. Purified human hematopoietic progenitor cells were grown in a serum-free medium: serum substitute BIT 9500 (Stem cell Technologies, Vancouver, BC, Canada). ..

Centrifugation:

Article Title: Antiviral activity of brincidofovir on parvovirus B19.
Article Snippet: .. PBMC, isolated by centrifugation in Ficoll-Paque Plus (GE Healthcare Bio-Sciences AB), were cultured in IMDM (Gibco) supplemented with 20% serum substitute BIT 9500 (StemCell Technologies), and enriched with erythropoietic growth factors as described (Bua et al., 2016; Filippone et al., 2010). ..

Recombinant:

Article Title: CD33-directed immunotherapy with third-generation chimeric antigen receptor T cells and gemtuzumab ozogamicin in intact and CD33-edited acute myeloid leukemia and hematopoietic stem and progenitor cells.
Article Snippet: The 293T cells were cultured in Iscove's Modified Dulbecco's Medium (IMDM; Thermo Fisher Scientific) containing 10% FBS. .. CD34-positive HSPCs were cultured in IMDM containing 20% serum substitute BIT 9500 (Stem Cell Technologies, Vancouver, Canada), 50 ng/mL recombinant human thrombopoietin (TPO; Miltenyi Biotec), 100 ng/ mL stem cell factor (SCF; Miltenyi Biotec), 100 ng/mL—FLT3-ligand (FLT3-L, Miltenyi Biotec), 100 μM beta-mercaptoethanol (Thermo Fisher Scientific), 50 μg/mL gentamicin (Carl Roth, Karlsruhe, Germany), 1 penicillin/streptomycin (pen/strep; Biochrom) and 35 nM UM171 (Miltenyi Biotec). .. AML blasts were cultured in IMDM (Thermo Fisher Scientific) containing 15% BIT 9500, 20 ng/mL interleukin (IL)-3 (Miltenyi Biotec), 20 ng/mL granulocyte colonystimulating factor (G-CSF) (Miltenyi Biotec), 50 ng/mL FLT3-L, 100 ng/mL SCF, 50 μg/mL gentamicin, 10 μg/mL ciprofloxacin (Carl Roth), 100 μM beta-Mercaptoethanol, 500 nM stemregenin 1 (SR1, Stem Cell Technologies) and 500 nM UM729 (Miltenyi Biotec).



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